Multiple novel and prevalent astroviruses in pigs
| Autor: | Marc-André Laurin, Margaux Dastor, Estelle Gallice, Stéphanie Roi, Yvan L’Homme, Zhiyao Luo |
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| Rok vydání: | 2011 |
| Předmět: |
food.ingredient
Swine viruses Molecular Sequence Data Cross-species transmission Zoology Genome Viral Biology Microbiology Article Astrovirus Feces Monophyly fluids and secretions food Phylogenetics Astroviridae Infections Genetic variation Prevalence Animals Phylogeny Taxonomy Swine Diseases Genetic diversity General Veterinary Phylogenetic tree Quebec Genetic Variation virus diseases Mamastrovirus General Medicine biology.organism_classification Virology Astroviridae Pigs Capsid Proteins |
| Zdroj: | Veterinary Microbiology |
| ISSN: | 0378-1135 |
| Popis: | Knowledge of porcine astrovirus diversity and epidemiology remains limited. We used a broad range PCR approach to investigate the presence and diversity of astroviruses in healthy pigs of different ages on 20 farms and in 3 slaughterhouses situated in the province of Quebec, Canada between 2005 and 2007. Our study unexpectedly revealed remarkable levels of genetic diversity and high prevalence of astroviruses in pigs of this province. Astroviruses were detected on every farm investigated and in all age groups of pigs, from suckling piglets to adults. In addition, we found that nearly 80% of healthy finisher pigs harbour astroviruses in their intestine at slaughter. Phylogenetic evidence based on partial polymerase and complete capsid sequences, suggests that porcine astroviruses do not form a monophyletic group but are rather found on separate branches across the mamastrovirus tree. In addition to type species strains, we found highly divergent strains that form two additional lineages, one of which falls outside existing taxonomic groups. The presence of diverse astroviruses in a majority of healthy pigs likely represents a continuous source of infection to piglets and possibly to other animal species including humans. Porcine astrovirus strains appeared phylogenetically related not only to prototypical human astroviruses, as was already known, but also to novel human strains recently discovered suggesting multiple cross species transmission events between these hosts and other animal species. Overall, the findings reported in this study suggest an active role of pigs in the evolution and ecology of the Astroviridae. |
| Databáze: | OpenAIRE |
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