Selective photodynamic inactivation of Helicobacter pylori by a cationic benzylidene cyclopentanone photosensitizer - an in vitro and ex vivo study
| Autor: | Shaona Zhou, Limei Xing, Xianghuan Guo, Ying Gu, Li-peng Zhang, Haixia Qiu, Ying Wang, Yuxia Zhao, Jing Zeng, Leili Wang, Yanyan Fang |
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| Rok vydání: | 2021 |
| Předmět: |
Cell Survival
medicine.medical_treatment Biophysics Photodynamic therapy Cyclopentanes Benzylidene Compounds Cell Line Helicobacter Infections Mice Cations Gastric mucosa medicine Animals Humans Radiology Nuclear Medicine and imaging Photosensitizer Photosensitizing Agents Radiation Helicobacter pylori Radiological and Ultrasound Technology biology Chemistry Lasers Antimicrobial biology.organism_classification Molecular biology In vitro Disease Models Animal medicine.anatomical_structure Gastric Mucosa Phototoxicity Ex vivo |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 223:112287 |
| ISSN: | 1011-1344 |
| Popis: | The rise in the antibiotic resistance rate of Helicobacter pylori has led to an increasing eradication failure of this carcinogenic bacterial pathogen worldwide. This underlines the need for alternative antibacterial strategies against H. pylori infection. Antimicrobial photodynamic therapy (aPDT) is a promising non-pharmacological antibacterial technology. In this study, the selective killing activities of three benzylidene cyclopentanone (BCP) photosensitizers (Y1, P1 and P3) towards H. pylori over normal human gastric epithelial GES-1 cells were evaluated and the ex vivo photodynamic inactivation effect was preliminarily assessed on twelve H. Pylor-infected mice. Results showed that under the irradiation of 24 J/cm2 532 nm laser, Y1, P1 and P3 at 2.5 μM induced a 3-log10 reduction of H. pylori CFU (99.9% killing). Confocal images showed that P3, unlike Y1 and P1, could not be uptaken by GES-1 cells. P3 at 2.5 to 20 μM showed not significant (p > 0.05) phototoxicity to GES-1 cells, nevertheless, Y1 and P1 under the same concentrations exhibited remarkable phototoxicity to GES-1 cells. In the co-culture of H. pylori and GES-1 cells, P3 at 2.5 μM led to a complete eradication of H. pylori under the irradiation of 24 J/cm2 532 nm laser. While for the GES-1 cells, no significant (p > 0.05) phototoxicity was observed under the same aPDT dosage. The ex vivo experiments showed that P3 mediated aPDT resulted in 82.4% to 100% reduction of H. pylori CFU without damaging the gastric mucosa. To sum up, P3 is a promising anti-H. pylori photosensitizer with the ability to selectively photo-inactivate H. pylori while sparing normal gastric tissues. |
| Databáze: | OpenAIRE |
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