Molecular characterization of Cystoisospora belli and unizoite tissue cyst in patients with Acquired Immunodeficiency Syndrome
| Autor: | Cecilia Di Risio, Jorge Néstor Velásquez, Agustín Víctor Chertcoff, Germán Astudillo Osvaldo, Silvana Carnevale, Gladys Elisabet Perissé, Cristina Etchart |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Diarrhea Male Pathology medicine.medical_specialty Duodenum Cystoisospora Molecular Sequence Data law.invention Young Adult Species Specificity law Biopsy medicine Humans Cyst Intestinal Diseases Parasitic Sida Polymerase chain reaction Acquired Immunodeficiency Syndrome Lamina propria AIDS-Related Opportunistic Infections Base Sequence biology medicine.diagnostic_test Coccidiosis Sequence Analysis DNA DNA Protozoan Middle Aged biology.organism_classification medicine.disease Infectious Diseases medicine.anatomical_structure Sarcocystidae Female Animal Science and Zoology Parasitology Cystoisospora belli Viral disease |
| Zdroj: | Parasitology. 138:279-286 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182010001253 |
| Popis: | SUMMARYCystoisospora belli is a coccidian protozoan that can cause chronic diarrhoea, acalculous cholecystitis and cholangiopathy in AIDS patients. We applied molecular methods to identify Cystoisospora at species level in AIDS patients presenting with and without the presence of unizoites in lamina propria. Coprological and histological analyses were performed in stool and/or biopsy samples from 8 Cystoisospora-infected patients. DNA from the same samples was used to amplify 2 fragments of the SSU-rRNA gene and the ITS-1 region. Sequencing of the resulting amplicons identified C. belli infections in all cases, independent of the presence or absence of unizoite tissue cysts. Further work should be considered in order to find molecular targets related to strain variations in C. belli. |
| Databáze: | OpenAIRE |
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