Molecular imaging can identify the location to perform a frozen biopsy during intraoperative frozen section consultation

Autor: Charuhas Deshpande, Lydia G. Frenzel-Sulyok, Mitchell G. Bryski, Leslie A. Litzky, Sunil Singhal, E. James Delikatny
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Fluorescence-lifetime imaging microscopy
Biopsy
030204 cardiovascular system & hematology
Pathology and Laboratory Medicine
Lung and Intrathoracic Tumors
chemistry.chemical_compound
Mice
0302 clinical medicine
Spectrum Analysis Techniques
Surgical oncology
Medicine and Health Sciences
Medicine
Frozen Sections
Multidisciplinary
medicine.diagnostic_test
Small footprint
Optical Imaging
near-Infrared Spectroscopy
Tail vein
Molecular Imaging
Laboratory Equipment
Oncology
030220 oncology & carcinogenesis
Engineering and Technology
Research Article
Imaging Techniques
Science
Equipment
Infrared Spectroscopy
Research and Analysis Methods
03 medical and health sciences
Signs and Symptoms
Malignant Tumors
Fluorescence Imaging
Cryostats
Animals
Humans
Colorectal Cancer
Frozen section procedure
business.industry
technology
industry
and agriculture

Cancers and Neoplasms
chemistry
Anatomical Pathology
Surgical Pathology
Lesions
Molecular imaging
Clinical Medicine
business
Nuclear medicine
Indocyanine green
Zdroj: PLoS ONE, Vol 16, Iss 6, p e0252731 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Background Intraoperative frozen section (FS) consultation is an important tool in surgical oncology that suffers from sampling error because the pathologist does not always know where to perform a biopsy of the surgical specimen. Intraoperative molecular imaging is a technology used in the OR to visualize lesions during surgery. We hypothesized that molecular imaging can address this pathology challenge in FS by visualizing the cancer cells in the specimen in the pathology suite. Here, we report the development and validation of a molecular-imaging capable cryostat called Smart-Cut. Methods A molecular imaging capable cryostat prototype was developed and tested using a murine model. Tumors grown in mice were targeted with a NIR contrast agent, indocyanine green (ICG), via tail vein injection. Tumors and adjacent normal tissue samples were frozen sectioned with Smart-Cut. Fluorescent sections and non-fluorescent sections were prepared for H&E and fluorescent microscopy. Fluorescent signal was quantified by tumor-to-background ratio (TBR). NIR fluorescence was tested in one patient enrolled in a clinical trial. Results The Smart-Cut prototype has a small footprint and fits well in the pathology suite. Fluorescence imaging with Smart-Cut identified cancerous tissue in the specimen in all 12 mice. No false positives or false negatives were seen, as confirmed by H&E. The mean TBR in Smart-Cut positive tissue sections was 6.8 (SD±3.8). In a clinical application in the pathology suite, NIR imaging identified two lesions in a pulmonary resection specimen, where traditional grossing only identified one. Conclusion Molecular imaging can be integrated into the pathology suite via the Smart-Cut device, and can detect cancer in frozen tissue sections using molecular imaging in a murine model.
Databáze: OpenAIRE