Global gene expression analysis using RNA-seq uncovered a new role for SR1/CAMTA3 transcription factor in salt stress
| Autor: | Denghui Xing, Anireddy S. N. Reddy, Amira A. E. Abdel-Hameed, K.V.S.K. Prasad |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Biology Genes Plant 01 natural sciences Article 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Gene expression Gene Silencing Transcription factor Gene Regulator gene Regulation of gene expression Genetics Multidisciplinary Binding Sites Arabidopsis Proteins Sequence Analysis RNA Gene Expression Profiling Promoter Salt Tolerance Biotic stress Gene expression profiling 030104 developmental biology Gene Ontology Seedlings Transcriptome 010606 plant biology & botany Transcription Factors |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep27021 |
| Popis: | Abiotic and biotic stresses cause significant yield losses in all crops. Acquisition of stress tolerance in plants requires rapid reprogramming of gene expression. SR1/CAMTA3, a member of signal responsive transcription factors (TFs), functions both as a positive and a negative regulator of biotic stress responses and as a positive regulator of cold stress-induced gene expression. Using high throughput RNA-seq, we identified ~3000 SR1-regulated genes. Promoters of about 60% of the differentially expressed genes have a known DNA binding site for SR1, suggesting that they are likely direct targets. Gene ontology analysis of SR1-regulated genes confirmed previously known functions of SR1 and uncovered a potential role for this TF in salt stress. Our results showed that SR1 mutant is more tolerant to salt stress than the wild type and complemented line. Improved tolerance of sr1 seedlings to salt is accompanied with the induction of salt-responsive genes. Furthermore, ChIP-PCR results showed that SR1 binds to promoters of several salt-responsive genes. These results suggest that SR1 acts as a negative regulator of salt tolerance by directly repressing the expression of salt-responsive genes. Overall, this study identified SR1-regulated genes globally and uncovered a previously uncharacterized role for SR1 in salt stress response. |
| Databáze: | OpenAIRE |
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