Gene expression in mouse spermatogenesis during ontogenesis
| Autor: | Enzo Vicari, Fabio D’Amico, Rosario D'Agata, Aldo E. Calogero, Fabrizio Romano, Franca Maria Pezzino, S Sanfilippo, M. R. Garofalo, Salvatore Travali, Maurizio Averna, Paola Castrogiovanni, Laura Litrico, Massimo Libra, Vincenza Giuffrida, Giorgio Nicotra, Rosa Imbesi |
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| Přispěvatelé: | GIUFFRIDA V, PEZZINO FM, ROMANO F, LITRICO L, GAROFALO MR, NICOTRA G, LIBRA M, D'AMICO F, CASTROGIOVANNI P, IMBESI R, AVERNA M, SANFILIPPO S, D'AGATA R, VICARI E, CALOGERO AE, TRAVALI S |
| Předmět: |
Male
Chaperonins Spermiogenesis Mouse testis ontogenesis Biology Mice Gene expression Testis Genetics medicine Animals RNA Messenger Spermatogenesis Gene Glycoproteins Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental Proteins General Medicine Cell cycle Molecular biology Cell biology Chromatin medicine.anatomical_structure beta 2-Glycoprotein I Apolipoprotein H Co-Repressor Proteins Germ cell gene expression mouse testis ontogenesis spermatogenesis Chaperonin Containing TCP-1 Transcription Factors |
| Zdroj: | Scopus-Elsevier |
| Popis: | In this study, we evaluated the expression of genes probably involved in spermatogenesis in the mouse. We examined cytosolic chaperonin theta subunit (CCTtheta), Ngg1 interacting factor 3 like 1 binding protein 1 (NIF3L1 BP1) and apolipoprotein H (ApoH) expression during mouse onto-geny using RT-PCR. Testicular tissue was obtained from mice 3, 6, 8, 10, 12, 14, 18, 20 and 40 (adult) days after birth. For each mouse, one testis was used for histological examination, whereas RNA was extracted from the controlateral testis for expression analysis. RT-PCR analysis showed that CCTtheta gene expression was low until day 10, but increased drastically afterwards. At this age, spermatocytes started to be present in the mouse testis. Therefore, CCT protein could be involved in chromatin packaging and remodeling during spermiogenesis, as also suggested by other studies. NIF3L1 BP1 expression increased steadily during ontogenesis reaching maximum levels in the adult mouse when all germ cell stages are present. This finding suggests that NIF3L1 BP1 is a gene not expressed by a specific germ cell type. ApoH expression was very low or absent during prepuberal stages, whereas it was detectable in the adult testis when spermatogenesis was completed. This suggests that ApoH may be involved in clearing apoptotic bodies during spermatogenesis since apoptotic events increase during spermatogenesis. This study contributes to understanding the role played by genes important for spermatogenesis. |
| Databáze: | OpenAIRE |
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