A detailed physical map of the porcine major histocompatibility complex (MHC) class III region: Comparison with human and mouse MHC class III regions
| Autor: | R. D. Campbell, Patrick Chardon, Marcel Vaiman, A. Van Zeveren, Yves Bouquet, Luc Peelman, A. Van De Weghe, Marc Mattheeuws |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Swine Molecular Sequence Data Locus (genetics) Major histocompatibility complex Major Histocompatibility Complex Mice chemistry.chemical_compound Cytochrome P-450 Enzyme System Species Specificity MHC class I Genetics Pulsed-field gel electrophoresis Homologous chromosome Animals Humans Gene Southern blot biology Chromosome Mapping Complement C4 RNA Nucleotidyltransferases chemistry biology.protein RNA Helicases DNA |
| Zdroj: | Mammalian Genome. 7:363-367 |
| ISSN: | 1432-1777 0938-8990 |
| Popis: | A detailed physical map of the porcine MHC class III region on Chr 7 was constructed with a panel of probes in a series of hybridizations on genomic pulsed field gel electrophoresis (PFGE) Southern blots. A precise organization of the 700-kb segment of DNA between G18 and BAT1 can now be proposed, with more than 30 genes mapped to it. Comparison of this region with homologous regions in human and mouse showed only minor differences. The biggest difference was observed in the CYP21/C4 locus with only one CYP21 gene and one C4 gene found, whereas in human and mouse these genes are duplicated. These results show the class III region is very well conserved between pig, human, and mouse, in contrast with the class I and class II regions, which seem more prone to rearrangements. |
| Databáze: | OpenAIRE |
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