Separate regions of an adenovirus E1B protein critical for different biological functions
| Autor: | Irene Mak, Stanley Mak |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA Replication
Gene Expression Regulation Viral viruses DNA Mutational Analysis Mutagenesis (molecular biology technique) Viral Plaque Assay Biology medicine.disease_cause Transfection Virus Replication Adenovirus E1B protein Virus Cell Line chemistry.chemical_compound Plasmid Virology medicine Humans Amino Acid Sequence Cell Line Transformed chemistry.chemical_classification Adenoviruses Human Adenovirus Early Proteins Oncogene Proteins Viral Cell Transformation Viral Amino acid Adenoviridae Cell Transformation Neoplastic Biochemistry chemistry Viral replication DNA Viral Electrophoresis Polyacrylamide Gel DNA Plasmids |
| Zdroj: | Virology. 176(2) |
| ISSN: | 0042-6822 |
| Popis: | The E1B region of Ad12 encodes two major proteins, the 482R (55K) and 163R (19K). In this report we showed that the E1B 482R is multifunctional, in that its structure may somehow contribute to its own stability, in viral DNA and virus replication, in transformation of primary cells, and in tumorigenicity. Deletion of the first 24 amino acids and of as residues 114–155 (d142) results in an instability of the 55K protein. The N-terminal 24 as residues (pml852) or amino acids residues 80–96 (d117) are not required for viral DNA or virus replication, whereas amino acid residues between 114 and 155 (d142) are absolutely necessary for viral DNA synthesis. Deletion of amino acid residues 1–24, 80–96, and 114–155 (d142) greatly reduces the transforming ability of both virus and plasmids containing any one of these deletions. One of the critical regions for tumorigenicity resides within amino acid residues 80–96, since cells transformed by this plasmid are nontumorigenic. On the other hand the region bounded by amino acid residues 114–155 (d142) is not required for tumorigenicity in immunocompetent animals. |
| Databáze: | OpenAIRE |
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