PrimerZ: streamlined primer design for promoters, exons and human SNPs
| Autor: | Cheng-Chih Huang, Kuo-Hsi Lee, Adam Yao, Ming-Fang Tsai, Yi-Jung Lin, Yuan-Tsong Chen, Yu-Chang Cheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
dbSNP
Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Exon Automation Web page Databases Genetic Genetics Web application Ensembl Humans RNA Messenger Promoter Regions Genetic Gene DNA Primers business.industry Computational Biology Promoter Articles Exons ComputingMethodologies_PATTERNRECOGNITION Genetic Techniques Primer (molecular biology) business Algorithms Software |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | PrimerZ (http://genepipe.ngc.sinica.edu.tw/primerz/) is a web application dedicated primarily to primer design for genes and human SNPs. PrimerZ accepts genes by gene name or Ensembl accession code, and SNPs by dbSNP rs or AFFY_Probe IDs. The promoter and exon sequence information of all gene transcripts fetched from the Ensembl database (http://www.ensembl.org) are processed before being passed on to Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi) for individual primer design. All results returned from Primer 3 are organized and integrated in a specially designed web page for easy browsing. Besides the web page presentation, csv text file export is also provided for enhanced user convenience. PrimerZ automates highly standard but tedious gene primer design to improve the success rate of PCR experiments. More than 2000 primers have been designed with PrimerZ at our institute since 2004 and the success rate is over 70%. The addition of several new features has made PrimerZ even more useful to the research community in facilitating primer design for promoters, exons and SNPs. |
| Databáze: | OpenAIRE |
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