Effects of Resveratrol on the Expression and DNA Methylation of Cytokine Genes in Diabetic Rat Aortas
| Autor: | Xudan Lou, Sven Skog, Shi-jin Xia, Haidong Wang, Jiao Sun |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Immunology Guanosine Resveratrol Biology Diabetes Mellitus Experimental Proinflammatory cytokine Rats Sprague-Dawley chemistry.chemical_compound Internal medicine Stilbenes Diabetes Mellitus medicine Animals Humans Immunology and Allergy Gene silencing Epigenetics Th1-Th2 Balance Aorta Anti-Inflammatory Agents Non-Steroidal General Medicine DNA Methylation Rats Blot Endocrinology Gene Expression Regulation chemistry DNA methylation Cytokines Immunohistochemistry Diabetic Angiopathies |
| Zdroj: | Archivum Immunologiae et Therapiae Experimentalis. 62:329-340 |
| ISSN: | 1661-4917 0004-069X |
| Popis: | This paper studies the expression of proinflammatory cytokines such as IL-1β, IL-6, TNF-α, and IFN-γ and anti-inflammatory cytokines such as IL-10 in diabetic rat aortas, the effects of resveratrol on these cytokines, and the potential epigenetic mechanisms involved. The experiment was performed on rats divided into four groups: normal group (NC), normal interventional group (NB), diabetic group (DM), and diabetic interventional group (DB). The NB and DB groups were treated with resveratrol. After more than 3 months, the rats’ aortas were removed and analyzed for cytokines by using immunohistochemistry, Western blotting, real-time PCR, and methylation-specific PCR. Histological localization of these cytokines was mainly found in the arterial intima of diabetic rats. The protein and mRNA expression levels of IL-1β, IL-6, TNF-α, and IFN-γ were significantly higher in the DM group than in the NC group (p |
| Databáze: | OpenAIRE |
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