Coadministration of Hedera helix L. Extract Enabled Mice to Overcome Insufficient Protection against Influenza A/PR/8 Virus Infection under Suboptimal Treatment with Oseltamivir
| Autor: | Yong Soo Kwon, Jae Hyoung Song, Sun Young Chang, Yeon-Jeong Kim, Hyun-Jeong Ko, Hyeon Gun Jeong, Hyun Pyo Kim, Eun Hye Hong, Aeri Shim, Bo Ra Lee, Hyuk Song, Jong Geal Kim, Sang-Uk Seo, Bo Eun Kwon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Oseltamivir
medicine.drug_class viruses lcsh:Medicine Neuraminidase Pharmacology In Vitro Techniques medicine.disease_cause Antiviral Agents Virus Proinflammatory cytokine chemistry.chemical_compound Mice Cytopathogenic Effect Viral Orthomyxoviridae Infections medicine Influenza A virus Animals Enzyme Inhibitors lcsh:Science Lung Cytopathic effect Inflammation Multidisciplinary Neuraminidase inhibitor biology Hedera Plant Extracts lcsh:R virus diseases Drug Synergism Saponins Virology respiratory tract diseases Mice Inbred C57BL chemistry biology.protein Cytokines lcsh:Q Drug Therapy Combination Antiviral drug Bronchoalveolar Lavage Fluid Research Article Phytotherapy |
| Zdroj: | PLoS ONE PLOS ONE(10): 6 PLoS ONE, Vol 10, Iss 6, p e0131089 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Several anti-influenza drugs that reduce disease manifestation exist, and although these drugs provide clinical benefits in infected patients, their efficacy is limited by the emergence of drug-resistant influenza viruses. In the current study, we assessed the therapeutic strategy of enhancing the antiviral efficacy of an existing neuraminidase inhibitor, oseltamivir, by coadministering with the leaf extract from Hedera helix L, commonly known as ivy. Ivy extract has anti-inflammatory, antibacterial, antifungal, and antihelminthic properties. In the present study, we investigated its potential antiviral properties against influenza A/PR/8 (PR8) virus in a mouse model with suboptimal oseltamivir that mimics a poor clinical response to antiviral drug treatment. Suboptimal oseltamivir resulted in insufficient protection against PR8 infection. Oral administration of ivy extract with suboptimal oseltamivir increased the antiviral activity of oseltamivir. Ivy extract and its compounds, particularly hedrasaponin F, significantly reduced the cytopathic effect in PR8-infected A549 cells in the presence of oseltamivir. Compared with oseltamivir treatment alone, coadministration of the fraction of ivy extract that contained the highest proportion of hedrasaponin F with oseltamivir decreased pulmonary inflammation in PR8-infected mice. Inflammatory cytokines and chemokines, including tumor necrosis factor-alpha and chemokine (C-C motif) ligand 2, were reduced by treatment with oseltamivir and the fraction of ivy extract. Analysis of inflammatory cell infiltration in the bronchial alveolar of PR8-infected mice revealed that CD11b+Ly6G+ and CD11b+Ly6Cint cells were recruited after virus infection; coadministration of the ivy extract fraction with oseltamivir reduced infiltration of these inflammatory cells. In a model of suboptimal oseltamivir treatment, coadministration of ivy extract fraction that includes hedrasaponin F increased protection against PR8 infection that could be explained by its antiviral and anti-inflammatory activities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|