Molecular Epidemiology of Salmonella enterica Serovar Typhimurium Isolates from Cattle in Hokkaido, Japan: Evidence of Clonal Replacement and Characterization of the Disseminated Clone
| Autor: | Satoru Tezuka, Yuuji Nakaoka, Ryoko Ishihara, Hizuru Okazaki, Masato Akiba, Sou-ichi Makino, Hironari Yamada, Takayuki Kubota, Toru Kanno, Hideki Hanyu, Eiji Hata, Ikuo Uchida, Yukino Tamamura, Kiyoshi Tanaka, Shinichi Hatama, Natstumi Kataoka, Masato Kishima |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Salmonella typhimurium Serotype Gene Transfer Horizontal Virulence Factors Tetracycline Molecular Sequence Data Cattle Diseases Public Health Microbiology Multiple Loci VNTR Analysis Biology Applied Microbiology and Biotechnology Microbiology Plasmid Japan Drug Resistance Bacterial Escherichia coli medicine Pulsed-field gel electrophoresis Animals Cluster Analysis Molecular Epidemiology Salmonella Infections Animal Ecology Molecular epidemiology Kanamycin Sequence Analysis DNA biology.organism_classification Virology Anti-Bacterial Agents Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Molecular Typing Salmonella enterica Conjugation Genetic Cattle Plasmids Food Science Biotechnology medicine.drug |
| Zdroj: | Applied and Environmental Microbiology. 77:1739-1750 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | The molecular epidemiology of 545 Salmonella enterica serovar Typhimurium isolates collected between 1977 and 2009 from cattle in Hokkaido, Japan, was investigated using pulsed-field gel electrophoresis (PFGE). Nine main clusters were identified from 116 PFGE patterns. Cluster I comprised 248 isolates, 243 of which possessed a sequence specific to definitive phage type 104 (DT104) or U302. The cluster I isolates were dominant in 1993 to 2003, but their numbers declined beginning in 2004. Beginning in 2002, an increase was observed in the number of cluster VII isolates, consisting of 21 PFGE patterns comprising 165 isolates. A total of 116 isolates representative of the 116 PFGE profiles were analyzed by multilocus variable-number tandem-repeat analysis (MLVA). Other than two drug-sensitive isolates, 19 isolates within cluster VII were classified in the same cluster by MLVA. Among the cluster VII isolates, an antibiotic resistance type showing resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, tetracycline, kanamycin, cefazolin, and sulfamethoxazole-trimethoprim and a resistance type showing resistance to ampicillin, streptomycin, sulfonamides, tetracycline, and kanamycin were found in 23 and 125 isolates, respectively. In the 19 isolates representative of cluster VII, the bla TEM-1 gene was found on a Salmonella serotype Typhimurium virulence plasmid, which was transferred to Escherichia coli by electroporation along with resistance to two to four other antimicrobials. Genomic analysis by subtractive hybridization and plasmid analysis suggested that the bla TEM-1 -carrying virulence plasmid has a mosaic structure composed of elements of different origin. These results indicate an emerging multidrug-resistant S . Typhimurium clone carrying a virulence-resistance plasmid among cattle in Hokkaido, Japan. |
| Databáze: | OpenAIRE |
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