Reduced spermatozoa functionality during stress is the consequence of adrenergic-mediated disturbance of mitochondrial dynamics markers
| Autor: | Isidora M. Starovlah, Silvana A. Andric, Tatjana S. Kostic, Sava M Radovic Pletikosic |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Epinephrine lcsh:Medicine Adrenergic Reproductive biology Mitochondrial Dynamics Article Immobilization In vivo Transcription (biology) medicine Animals Testosterone Rats Wistar lcsh:Science Receptor Hydrocortisone Multidisciplinary Kinase Chemistry urogenital system Gene Expression Profiling lcsh:R Dihydrotestosterone Spermatozoa Cell biology Mitochondria Rats Receptors Adrenergic lcsh:Q Corticosterone Ex vivo Biomarkers Stress Psychological medicine.drug Hormone Cell signalling |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Here we investigate the stress-signaling responsible for the effects of acute/repeated psychological stresses (the most common stresses in human society) on spermatozoa number and functionality, as well as the transcriptional profile of mitochondrial dynamics markers by using the in vivo and ex vivo approaches. Acute and repeated stress inhibit spermatozoa functionality (acute –> 3.2-fold, repeated –> 2.5-fold), while only repeated stress reduces the spermatozoa number (1.7-fold). Stress hormones mimic these effects and decrease the spermatozoa functionality (adrenaline: 10 µM –> 2.4-fold, 100 µM – > 2.8-fold; hydrocortisone: 50 pM –> 2.7-fold, 500 pM –> 8.5-fold). They also significantly disturb the transcriptional profile of all main mitochondrial dynamics markers in spermatozoa. Ex vivo manipulation of stress signaling in spermatozoa reveals that most of these effects are mediated through ɑ1-and/or-β-adrenergic receptors. The transcription of these receptors and their kinases in the same samples is under the significant influence of adrenergic signaling. Our results are the first to show the importance of mitochondrial dynamics markers in spermatozoa since the transcriptional profiles of sixteen-out-of-ninteen are disturbed by manipulation of stress-hormones-signaling. This is a completely new molecular approach to assess spermatozoa functionality and it is important for a better understanding of the correlations between stress, environmental-life-style and other factors, and male (in)fertility. |
| Databáze: | OpenAIRE |
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