Assay of the Immune Inhibitor in Classic Haemophilia: Application of Virus-Antibody Reaction Kinetics

Autor:	Judith G. Pool, Rupert G. Miller
Rok vydání:	1972
Předmět:	Factor VIII biology Chemistry Poliovirus Kinetics Hematology Hemophilia A medicine.disease_cause Antiviral Agents Virology Molecular biology Antibodies Virus Neutralization Chemical kinetics Immune system Antigen Neutralization Tests medicine biology.protein Humans Antigens Antibody
Zdroj:	British Journal of Haematology. 22:517-528
ISSN:	1365-2141 0007-1048
DOI:	10.1111/j.1365-2141.1972.tb05699.x
Popis:	Summary The kinetics of virus neutralization by antibody are shown to apply to factor-VIII neutralization by the immune antibody of haemophilic patients. The analysis established for virus neutralization has been used as a model for designing a general system for inhibitor assay based on mixtures in which antigen is present in excess, rather than with antibody in excess, as has been the case with systems previously proposed. The new system permits conversion of inhibitor potency results from other systems based on the use of different mixtures of factor VIII and inhibitor as long as factor VIII is in excess and as long as constant physical conditions of time and temperature of incubation are applied. It also takes account of the fact that some antibodies reveal a‘residual resistant fraction’ of antigen with which they cannot react. The procedure for converting assay data into‘Oxford units’ is described.
Databáze:	OpenAIRE
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