Expression and subcellular analyses of CCR8a/b genes with the identification of response to SGIV viral infect in orange-spotted grouper (Epinephelus coioides)
| Autor: | Qiwei Qin, Yanchuang Duan, Fangmei Lin, Zhi Guang, Li Wang, Jingxin Zhou, Keqi Li, Min Yang, Yuxin Wang, Qing Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Fish Proteins Orange-spotted grouper Aquatic Science Biology Receptors CCR8 Iridovirus 03 medical and health sciences Chemokine receptor Fish Diseases Interferon Gene expression medicine Environmental Chemistry Animals Grouper Gene 04 agricultural and veterinary sciences General Medicine biology.organism_classification Molecular biology DNA Virus Infections Perciformes 030104 developmental biology Viral replication 040102 fisheries 0401 agriculture forestry and fisheries CC chemokine receptors medicine.drug |
| Zdroj: | Fishshellfish immunology. 106 |
| ISSN: | 1095-9947 |
| Popis: | Chemokine receptors are a superfamily of seven transmembrane domain G-coupled receptors, and they play important roles in immune surveillance, inflammation, and development. Recently, nine CC chemokine receptors (CCRs) were identified and cloned from orange-spotted grouper (Epinephelus coioides) and annotated by phylogenetic and syntenic analyses. We detected mRNA transcripts for CCRs in healthy tissues of E. coioides, and CCR genes were highly expressed in the immune-relevant tissues. Analysis of gene expression after Singapore grouper iridovirus (SGIV) infection indicated that CCR genes are regulated in a gene-specific manner. CCR8a and CCR8b were significantly upregulated in the spleen and liver of resistant fish, indicating potential roles in immunity against the pathogen. Fluorescence microscopy revealed that CCR8a and CCR8b were expressed predominantly in the cytoplasm. Overexpression of CCR8a and CCR8b in grouper cells significantly inhibited the replication of SGIV, demonstrating that they delayed the occurrence of cytopathic effects induced by SGIV infection and inhibited viral gene transcription. CCR8a and CCR8b overexpression also significantly increased the expression of interferon (IFN)-related cytokines and activated IFN response element and IFN promoter activities. These results demonstrated that CCR8a and CCR8b might have an antiviral function against SGIV infect. |
| Databáze: | OpenAIRE |
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