Semiconstant-Time P,H-COSY NMR: Analysis of Complex Mixtures of Phospholipids Originating from Helicobacter pylori
| Autor: | Anna Arnqvist, Katja Petzold, Jürgen Schleucher, Gerhard Gröbner, Annelie Olofsson |
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| Rok vydání: | 2009 |
| Předmět: |
Cardiolipins
Phospholipid Biochemistry Catalysis chemistry.chemical_compound Colloid and Surface Chemistry medicine Nuclear Magnetic Resonance Biomolecular Phospholipids Helicobacter pylori biology Chemistry Phosphatidylethanolamines Chemical shift Cell Membrane Phosphatidylglycerols General Chemistry medicine.disease biology.organism_classification Membrane composition Membrane Peptic ulcer Phosphatidylcholines Two-dimensional nuclear magnetic resonance spectroscopy |
| Zdroj: | Journal of the American Chemical Society. 131:14150-14151 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja905282h |
| Popis: | Lipids play a central role in numerous biological events, ranging from normal physiological processes to host-pathogen interactions. The proposed semiconstant-time (31)P,(1)H-COSY NMR experiment provides identification of known and structural characterization of unknown phospholipids in complex membrane extracts with high sensitivity, based on the combination of their (1)H and (31)P chemical shifts and coupling patterns. Furthermore, the spectra allow quantification of phospholipid composition. Analysis of the phospholipid composition of Helicobacter pylori, the causative agent of peptic ulcer disease, showed the presence of uncommon phospholipids. This novel NMR approach allows the study of changes in membrane composition in response to biological stimuli and opens up the possibility of identifying soluble phosphorus species in a number of research fields. |
| Databáze: | OpenAIRE |
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