Cardioprotective effects of a Fructus Aurantii polysaccharide in isoproterenol-induced myocardial ischemic rats
| Autor: | Zihe Ding, Yi Wang, Zunpeng Shu, Zhao Hong, Tianyi Xia, Yanni Yang, Renxing Zhong, Wujing Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
MAPK/ERK pathway Citrus Cardiotonic Agents Antioxidant NF-E2-Related Factor 2 medicine.medical_treatment Myocardial Ischemia 02 engineering and technology Pharmacology medicine.disease_cause Biochemistry Antioxidants Rats Sprague-Dawley Phosphatidylinositol 3-Kinases 03 medical and health sciences Polysaccharides Structural Biology medicine Animals Molecular Biology Protein kinase B PI3K/AKT/mTOR pathway 030304 developmental biology 0303 health sciences Plant Extracts Chemistry Isoproterenol General Medicine Adrenergic beta-Agonists 021001 nanoscience & nanotechnology Rats Blot Disease Models Animal Oxidative Stress Apoptosis Phosphorylation 0210 nano-technology Oxidative stress Signal Transduction |
| Zdroj: | International Journal of Biological Macromolecules. 155:995-1002 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.11.063 |
| Popis: | CALB-3, a purified acidic hetero-polysaccharide isolated from Fructus aurantii, has been shown to exert cardioprotective effects in vitro. Recently, we investigated the protective effects of CALB-3 on myocardial injury and its possible mechanisms of action using a rat model of myocardial ischemia. In this study, a myocardial ischemia model was established via intragastric administration of 2 mg/kg isoproterenol (ISO) to male Sprague–Dawley rats (200–220 g) daily for 3 days. We found that pretreatment with CALB-3 (50, 100, and 200 mg/kg, i.g.) daily for 21 days prevented ISO-induced myocardial damage, including improvement in electrocardiographic parameters, and decrease in serum cardiac enzymes, heart vacuolation, and TUNEL-positive cells. We used western blotting to identify the underlying mechanisms and determine the possible signal pathways involved. We found that CALB-3 pretreatment prevented apoptosis, increased the expression of antioxidant enzymes, and enhanced the binding of Nrf2 to the antioxidant response element. In addition, CALB-3 activated the phosphorylation of PI3K/Akt and ERK to increase the cytoprotective effect. Overall, our results show that CALB-3 is a promising polysaccharide for protecting against myocardial injury induced by ISO. |
| Databáze: | OpenAIRE |
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