Genome Sequence of Clostridium paraputrificum 373-A1 Isolated in Chile from a Patient Infected with Clostridium difficile
| Autor: | Fernando Díaz-Yáñez, Ángela Plaza-Garrido, Eduardo Castro-Nallar, Enzo Guerrero-Araya, Daniel Paredes-Sabja, Fernando Gil, Claudio Meneses, Marjorie Pizaro-Guajardo, Sandro L. Valenzuela |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Whole genome sequencing Strain (biology) Biology Gut flora Clostridium difficile medicine.disease biology.organism_classification Genome Microbiology 03 medical and health sciences 030104 developmental biology Bacteremia Genetics medicine Prokaryotes Molecular Biology Clostridium paraputrificum |
| Zdroj: | Genome Announcements Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 2169-8287 |
| Popis: | Clostridium paraputrificum is a Gram-positive, endospore-forming, strictly anaerobic, and chitinolytic bacterium (1). Few studies have addressed features of C. paraputrificum. Early work associated C. paraputrificum with an increased risk of colon cancer (2). C. paraputrificum has also been isolated from healthy individuals (3), as well as from cases of bacteremia (4) and patients with AIDS (5). C. paraputrificum contributes to colonic epithelium maturation and development (6); however, its role in infectious diseases remains unclear. Genomic data for strain 373-A1 was generated using Illumina technology (7) on an Illumina MiSeq platform (600 incorporated cycles; 2 × 300 bp). Library preparation was carried out according to the TruSeq DNA kit, generating 3,165,742 paired-end reads and 1.47 GB of information. Next, raw data were filtered (>Q20) and merged (minimum overlapping of 20 bp) in PEAR version 0.9.8 (8). The resulting set of reads was recleaned using Pathoscope version 2.0.6 (9) in order to obtain only the reads mapping against bacterial genomes. Data were assembled using SPAdes version 3.5.0 (10) with an auto cutoff coverage. The final assembly contained 41 contigs, and an N50 equal to 259,466 bp with a mean of coverage of 472×. The genome annotation was performed with the NCBI Prokaryotic Genome Annotation Pipeline (11) and this annotation was saved in GenBank (MAPZ01). The assembly of the draft genome sequence consists of 41 contigs amounting to 3,488,595 bp with a G+C content of 29.8%. Of the 3,351 predicted genes, 3,220 were protein-coding genes, and 106 RNAs and 24 rRNA operons were identified. The majority of the protein-coding genes (60.19%) were assigned a putative function, while the remaining ones were annotated as hypothetical proteins. For a comparison, the assembly report ASM42402v1 (C. paraputrificum AGR2156) was used. The genome of C. paraputrificum 373-A1 is smaller than C. paraputrificum AGR2156 (i.e., 3,488,595 bp versus 3,561,289 bp, respectively). C. paraputrificum 373-A1 had fewer predicted genes and genes encoding putative proteins (i.e., 3,351 genes and 3,220 proteins) than C. paraputrificum AGR2156 (i.e., 3,457 genes and 3,345 proteins). On the other hand, the G+C content was similar in both strains, with 29.8% and 29.6% for C. paraputrificum 373-A1 and C. paraputrificum AGR2156, respectively. When we focus on the number of contigs and N50 values, C. paraputrificum 373-A1 has 41 contigs with an N50 value of 259,466, in contrast with C. paraputrificum AGR2156, which has 32 contigs with an N50 value of 320,228, which indicates the high quality of our genome sequence. This draft sequence provides a new repertoire of genes and genome information for this strain of the opportunistic C. paraputrificum. |
| Databáze: | OpenAIRE |
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