Standardized BACTEC Method To Measure Clarithromycin Susceptibility of Mycobacterium genavense
| Autor: | Carolyn K. Wallis, Marie B. Coyle, La Donna C. Carlson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Microbiology (medical)
Serial dilution Mycobacterium genavense Microbial Sensitivity Tests Microbiology Mycobacterium Minimum inhibitory concentration Clarithromycin parasitic diseases medicine Humans Mycobacterium xenopi Antibacterial agent Mycobacterium Infections biology Reproducibility of Results Mycobacteriology and Aerobic Actinomycetes Hydrogen-Ion Concentration biology.organism_classification bacterial infections and mycoses Anti-Bacterial Agents Culture Media Mycobacterium simiae medicine.drug |
| Popis: | A standardized clarithromycin susceptibility test for Mycobacterium genavense is reported. The BACTEC radiometric broth dilution test method recommended for Mycobacterium avium complex was modified to develop a reliable and reproducible procedure. Test development involved optimization of medium pH and inoculum densities for antibiotic vials as well as growth control vials. MIC control organisms included Mycobacterium simiae , Mycobacterium avium , and Mycobacterium xenopi . Growth control vials required two to three inoculum dilutions, which varied for each species. Clarithromycin MICs and MBCs for 12 isolates and 1 colonial variant of M. genavense ranged from ≤0.06 to 0.25 μg/ml. |
| Databáze: | OpenAIRE |
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