Improved catalytic activity of a novel aspartate kinase by site-directed saturation mutagenesis

Autor:	Zhanqing Fan, Li Fang, Limei Wu, Zheren Wang, Yanan Wang, Caijing Han, Xiaoting Liu
Rok vydání:	2022
Předmět:	Aspartic Acid Kinetics Mutagenesis Mutagenesis Site-Directed Bioengineering Aspartate Kinase General Medicine Biotechnology
Zdroj:	Bioprocess and Biosystems Engineering. 45:541-551
ISSN:	1615-7605 1615-7591
Popis:	This study aimed to improve the catalytic activity of aspartate kinase (AK), the first key rate-limiting enzyme in the aspartic acid metabolism pathway, by site-directed saturation mutagenesis, and to weaken the synergistic feedback inhibition of metabolites and analyze its mechanism using molecular dynamics simulation (MD). The key residual sites around the inhibitor lysine (Lys) were selected to construct the mutant strains. The mutant A380M with significantly increased enzyme activity was obtained through enzyme activity screening. Kinetic analysis showed that the V
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6262ff43c0baa9d61592381ae452999 https://doi.org/10.1007/s00449-021-02677-6 Zobrazit plný text záznamu Full text from SpringerLink