Interferon-alpha-2b downregulation of oncogenes H-ras, c-raf-2, c-kit, c-myc, c-myb and c-fos in eskol, a hairy cell leukemic line, results in temporal perturbation of signal transduction cascade
| Autor: | L R Lowe, Steven T. Kosak, J C Sheaffer, N A Heerema, William H. Harvey, Omar S. Harb |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Cancer Research Cellular differentiation Cell Genes myc Down-Regulation Interferon alpha-2 Protein Serine-Threonine Kinases Biology Proto-Oncogene Proteins c-myb Downregulation and upregulation Proto-Oncogene Proteins Receptors Colony-Stimulating Factor Tumor Cells Cultured medicine Humans RNA Neoplasm Aged Leukemia Hairy Cell Oncogene Gene Expression Regulation Leukemic Genes fos Interferon-alpha Nucleic Acid Hybridization Receptor Protein-Tyrosine Kinases Oncogenes Hematology Blotting Northern Virology Molecular biology Recombinant Proteins Proto-Oncogene Proteins c-raf Proto-Oncogene Proteins c-kit Genes ras medicine.anatomical_structure Oncology Cell culture Karyotyping Signal transduction Signal Transduction |
| Zdroj: | Leukemia Research. 18:577-585 |
| ISSN: | 0145-2126 |
| Popis: | ESKOL, a B-lymphoblastoid cell line consisting of late differentiated cells, resembles hairy cell leukemia (HCL). It is pseudodiploid with a deleted 7q and an unbalanced translocation between chromosomes 4 and 6. It was screened by Northern hybridization for oncogenes, including H-ras, c-raf-2 (c-raf1p1), c-kit, c-myc, c-myb, c-fos, Fim-1, c-jun, ski, and c-mos, which are believed to contribute to B-cell differentiation and maturation. Interferon-alpha-2b (IFN) downregulates the expression of H-ras, c-raf-2, c-kit, c-myc, c-myb, c-fos, as determined by Northern hybridization of RNA isolated from cells harvested at time points during a 30 h time course. Downregulation of oncogenes H-ras, c-raf-2, c-kit, whose proteins are associated with cell surfaces or are cytosolar transducers, occurs before those oncogenes c-myc, c-myb, and c-fos, whose products are DNA binding proteins. This suggests a temporal perturbation of signal transduction by IFN. No change in oncogene expression occurred in non-treated cells nor were these oncogenes expressed in the non-transformed B-lymphoblast cell line, Wil-2, under the same treatment regimen. The basis for the IFN perturbation is not understood; yet the role of these oncogenes as signal transducers in differentiation and proliferation of human hematopoietic progenitors is unfolding, and ESKOL is an excellent system in which to study this phenomenon. |
| Databáze: | OpenAIRE |
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