Exploring the Mechanisms of Multiple Insecticide Resistance in a Highly Plasmodium-Infected Malaria Vector Anopheles Funestus Sensu Stricto from Sahel of Northern Nigeria
| Autor: | Williams Tchapga, Amen N. Fadel, Sulaiman S. Ibrahim, Helen Irving, Abdullahi Muhammad, Charles S. Wondji, Muhammad Mukhtar, F Sarkinfada, Jack Hearn, Jacob M. Riveron |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine lcsh:QH426-470 030231 tropical medicine Population malaria Indoor residual spraying Bendiocarb 119F mutation wa_395 Biology wc_765 resistance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Anopheles funestus metabolic parasitic diseases qx_600 Genetics medicine education Genetics (clinical) education.field_of_study wa_240 Plasmodium falciparum medicine.disease biology.organism_classification wc_750 lcsh:Genetics 030104 developmental biology Deltamethrin chemistry qx_650 qx_510 Vector (epidemiology) qx_135 qx_515 GSTe2 Malaria Permethrin medicine.drug |
| Zdroj: | Genes, Vol 11, Iss 454, p 454 (2020) Genes Volume 11 Issue 4 |
| ISSN: | 2073-4425 |
| Popis: | The Nigerian Government is scaling up the distribution of insecticide-treated bed nets for malaria control, but the lack of surveillance data, especially in the Sudan/Sahel region of the country, may hinder targeting priority populations. Here, the vectorial role and insecticide resistance profile of a population of a major malaria vector Anopheles funestus sensu stricto from Sahel of Nigeria was characterised. An. funestus s.s. was the only vector found, with a high human blood index (100%) and a biting rate of 5.3/person/night. High Plasmodium falciparum infection was discovered (sporozoite rate = 54.55%). The population is resistant to permethrin (mortality = 48.30%, LT50 = 65.76 min), deltamethrin, DDT (dichlorodiphenyltrichloroethane) and bendiocarb, with mortalities of 29.44%, 56.34% and 54.05%, respectively. Cone-bioassays established loss of efficacy of the pyrethroid-only long-lasting insecticidal nets (LLINs) but 100% recovery of susceptibility was obtained for piperonylbutoxide (PBO)-containing PermaNet® 3.0. Synergist bioassays with PBO and diethyl maleate recovered susceptibility, implicating CYP450s (permethrin mortality = 78.73%, &chi 2 = 22.33, P < 0.0001) and GSTs (DDT mortality = 81.44%, &chi 2 = 19.12, P < 0.0001). A high frequency of 119F GSTe2 mutation (0.84) was observed (OR = 16, &chi 2 = 3.40, P = 0.05), suggesting the preeminent role of metabolic resistance. These findings highlight challenges associated with deployment of LLINs and indoor residual spraying (IRS) in Nigeria. |
| Databáze: | OpenAIRE |
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