Heterogeneity in kinesin function
| Autor: | Changbong Hyeon, Michael Vershinin, Marvin E. Tanenbaum, Suvranta K. Tripathy, Jing Xu, Babu J.N. Reddy, Michelle K. Mattson-Hoss, Tory Doolin, Karim Arabi, Steven P. Gross, Dail Chapman |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Stochastic variation Homogeneous function Kinesins Biology Molecular Dynamics Simulation kinesin Biochemistry molecular motors Quantitative Biology::Subcellular Processes 03 medical and health sciences symbols.namesake Motion 0302 clinical medicine Protein Domains Microtubule Structural Biology Cell Line Tumor proteins in glycerol and altered pH Molecular motor Genetics Journal Article Animals Drosophila Proteins Humans Molecular Biology Function (mathematics) Cell Biology temperature dependence of kinesin motility Cell biology 030104 developmental biology Structural biology Homogeneous symbols Kinesin Drosophila Biological system 030217 neurology & neurosurgery kinesin velocity heterogeneity |
| Zdroj: | Traffic, 18(10), 658. Blackwell Munksgaard Traffic, 18(10), 658-671. Blackwell Munksgaard |
| ISSN: | 1398-9219 |
| DOI: | 10.1111/tra.12504 |
| Popis: | The kinesin family proteins are often studied as prototypical molecular motors; a deeper understanding of them can illuminate regulation of intracellular transport. It is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors’ velocities in vivo and in vitro is larger than the stochastic variation expected for an ensemble of “identical” motors. When moving on microtubules, slow and fast motors are persistently slow, and fast, respectively. We develop theory that provides quantitative criteria to determine whether the observed single-molecule variation is too large to be generated from an ensemble of identical molecules. To analyze such heterogeneity, we group traces into homogeneous sub-ensembles. Motility studies varying the temperature, pH and glycerol concentration suggest at least 2 distinct functional states that are independently affected by external conditions. We end by investigating the functional ramifications of such heterogeneity through Monte-Carlo multi-motor simulations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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