| Popis: |
1. 1. The recovery of Pasteurella tularensis cells suspended in a solution of gelatin-saline depended on ( a ) the rate of cooling, ( b ) the minimum temperature attained, ( c ) the time at a given temperature, and ( d ) the rate of subsequent warming. 2. 2. When cells were cooled to no lower than −30 °C., survival was comparatively high regardless of rate of cooling, exposure time, or rate of warming. 3. 3. When cells were cooled to −45 °C. or below, the slower the cooling and the more rapid the warming, the higher the recovery. 4. 4. The results are consistent with the view that death was associated primarily with slow warming or melting of intracellular ice. |
