The hOCT1 SNPs M420del and M408V alter imatinib uptake and M420del modifies clinical outcome in imatinib-treated chronic myeloid leukemia
| Autor: | Triantafilos Liloglou, Tessa L. Holyoake, Munir Pirmohamed, George Xinarianos, Sudeep Pushpakom, Jieying-Eunice Zhang, Richard E. Clark, Letizia Foroni, Lihui Wang, Gemma Austin, Andrea L. Jorgensen, Martin C. Müller, Andrea Davies, Athina Giannoudis, Panagiotis D. Kottaridis |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Models Molecular Genotype Protein Conformation Immunology Gene Expression Antineoplastic Agents Single-nucleotide polymorphism Polymorphism Single Nucleotide Biochemistry Piperazines Young Adult Cell Line Tumor Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans SNP Protein Kinase Inhibitors Alleles Aged Aged 80 and over Gene Expression Regulation Leukemic business.industry Organic Cation Transporter 1 Myeloid leukemia Imatinib Cell Biology Hematology Transfection Middle Aged Pyrimidines Treatment Outcome Imatinib mesylate Benzamides Imatinib Mesylate Cancer research Pyrosequencing Female Primer (molecular biology) business medicine.drug |
| Zdroj: | Blood. 121:628-637 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2012-01-405035 |
| Popis: | Although the prognosis of chronic myeloid leukemia (CML) patients treated with imatinib is good, many fail to develop an optimal response or lose one. This heterogeneity could be attributed to the presence of human organic cation transporter-1 (hOCT1) single nucleotide polymorphisms (SNPs). In the present study, we analyzed the effect of 23 hOCT1 SNPs on imatinib treatment outcome in newly diagnosed CML patients using MassARRAY sequencing and pyrosequencing. The only SNP associated with outcome was M420del (rs35191146), with patients with the M420del demonstrating an increased probability of imatinib treatment failure. In CML cell lines transfected with M420del and/or M408V, M420del significantly decreased imatinib uptake, but this effect was countered if the M408V (rs628031) SNP was also present. A similar effect was seen for the uptake of the hOCT1 substrates TEA+ and ASP+. Finally, apparent hOCT1 mRNA levels were studied using both our earlier primers covering the M420del and another set that did not. Different mRNA expression was observed, explaining the disparity in published data on the prognostic importance of hOCT1 mRNA and highlighting the importance of avoiding common SNP sites in primer design. These data demonstrate that the common M420del SNP can modulate the outcome of imatinib treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|