Wolbachia detection in insects through LAMP: loop mediated isothermal amplification
| Autor: | Nilton Barnabé Rodrigues, Luciano Andrade Moreira, Daniela da Silva Gonçalves, Caroline Dantas de Oliveira, Anna Paula Alvim Cassimiro |
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| Rok vydání: | 2014 |
| Předmět: |
LAMP-loop mediated isothermal amplification
Insecta Time Factors biology Short Report Loop-mediated isothermal amplification Nucleic acid amplification technique biology.organism_classification Dna amplification Sensitivity and Specificity Virology Infectious Diseases Mosquito Biological Control Agents Parasitology parasitic diseases Animals Wolbachia Insect Nucleic Acid Amplification Techniques |
| Zdroj: | Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Background The bacterium Wolbachia is a promising agent for the biological control of vector-borne diseases as some strains have the ability to block the transmission of key human disease-causing pathogens. Fast, accurate and inexpensive methods of differentiating between infected and uninfected insects will be of critical importance as field-based trials of Wolbachia-based bio-control become increasingly common. Findings We have developed a specific and sensitive method of detecting Wolbachia based on the isothermal DNA amplification. This technique can be performed in an ordinary heat block without the need for gel-based visualisation, and is effective for a wide variety of insect hosts. Conclusion Here we present the development of a rapid, highly sensitive and inexpensive method to detect Wolbachia in a variety of insect hosts, including key mosquito disease vectors. |
| Databáze: | OpenAIRE |
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