Changes in anion permeability following hypotonic challenge in rat brain endothelial cells: different responses in primary cultures and in immortalised RBE4 cells
| Autor: | Margery A. Barrand, F.A Roux, K.A Wright, S Seetharaman, S. F. von Weikersthal |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Anions
Cell type Primary culture Cell Membrane Permeability Biophysics Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Structural Biology Functional expression Genetics Animals ATP Binding Cassette Transporter Subfamily B Member 1 Molecular Biology 030304 developmental biology Cell Line Transformed Cerebral Cortex 0303 health sciences Iodide efflux Microcirculation Transporter Cell Biology Iodides Rat brain Molecular biology Cell biology Rats Kinetics Hypotonic Solutions Verapamil Permeability (electromagnetism) Vincristine Cyclosporine Tonicity Anion permeability Efflux Endothelium Vascular 030217 neurology & neurosurgery Immortalised rat brain endothelial cells |
| Zdroj: | FEBS letters. 417(3) |
| ISSN: | 0014-5793 |
| Popis: | Hypotonicity-induced anion permeability changes were investigated but not detected in immortalised (RBE4) rat brain endothelial cells using iodide efflux measurements. Large, rapid increases were however observed in primary cultured cells. Both cell types were reinvestigated following culture in a common growth factor-depleted medium. Responses were still undetectable in the immortalised RBE4 cells. Reduced responses were observed in the primary cultured cells that also showed altered morphology and decreased activity of another transporter, P-glycoprotein. Thus both immortalisation and different culture conditions may alter functional expression in these cells of transporters involved in hypotonicity-induced anion permeability changes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text