Production of glucose-rich enzymatic hydrolysates from cellulosic pulps

Autor: Kamila Przybysz Buzała, Halina Kalinowska, Justyna Rosicka-Kaczmarek, Piotr Przybysz
Rok vydání: 2014
Předmět:
Zdroj: Cellulose. 22:663-674
ISSN: 1572-882X
0969-0239
Popis: The digestibility of cellulosic pulps derived by the sulfate process was assessed using commercial multienzyme preparations. Poplar wood pulps of relatively low lignin contents (Kappa numbers of 15.4–24.2), birch, beech and pine wood pulps (Kappa numbers of 25.8–31.4), and wheat straw pulp (Kappa number of 29.5) were efficiently hydrolyzed by a commercial preparation NS-22086 from Novozymes, containing cellulases and xylanases. At around 1.3 % (w/w) substrate concentration, yields of glucose from the poplar pulps were around 80 % on a dry weight (d.w.) basis while for the other four pulps they varied between approximately 70 % (for pine pulp) and 78 % d.w. (for beech and wheat straw pulps). At around 7.4 % (w/w) poplar pulp (Kappa number of 24.2) concentration, glucose yield was around 61 % d.w. The NS-22086 preparation almost completely saccharified fines from a paper mill (around 74 % glucose yields on a dry weight basis) while digestion of poplar chips (particle size of 1.6–2.0 mm) and wheat straw chaff (particle size up to 6 mm) yielded around 5.3 and 14 % d.w. glucose, respectively (total reducing sugars yields of around 16 and 23 % d.w., respectively). These results show that plant biomass may be efficiently converted to glucose-rich hydrolysates by a two-step processing, consisting of kraft pulping followed by treatment with endo- and exo-type cellulases and hemicellulases. Glucose-rich hydrolysates may be also obtained by enzymatic digestion of fines from paper mills.
Databáze: OpenAIRE
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