An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples
| Autor: | Wojciech Łuczaj, Giuseppe Poli, Martina Podborska, Cinzia Mascia, Nicolle Breusing, Corinne M. Spickett, Norsyahida Mohd Fauzi, Ingrid Wiswedel, Tilman Grune, Maria Cristina Munteanu, Anca Dinischiotu, Rosario Casillas, Daniela Gradinaru, Sieglinde Zelzer, Antonín Lojek, Laura Bravo, Suzana Borović, Maria Teresa Mitjavila, Neven Zarkovic, Fiorella Biasi, Juan Gambini, Heidemarie Faber, Lidija Mrakovcic, Grzegorz Bartosz, Isidre Casals, Raquel Mateos, Luka Andrisic, Elżbieta Skrzydlewska, Jose Viña, Andreas Meinitzer, Paulina Sicinska, Joanna Drzewinska, Mustafa Atalay, Agnieszka Gajewska, Denisa Margina, Tarja Kokkola |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Ultraviolet Rays
Clinical Chemistry Tests Enzyme-Linked Immunosorbent Assay Isoprostanes medicine.disease_cause F2-isoprostanes Sensitivity and Specificity Biochemistry High-performance liquid chromatography Mass Spectrometry 4-Hydroxynonenal Lipid peroxidation Plasma chemistry.chemical_compound In vivo Malondialdehyde medicine Humans Chromatography High Pressure Liquid Aldehydes Chromatography Chemistry Reproducibility of Results oxidative stress F2-Isoprostanes 4.-hydroxynonenal malondialdehyde General Medicine Oxidative stress 4-hydroxynonenal Lipid Peroxidation Chromatography Liquid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F2-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity. © 2010 Informa UK, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|