Prevalence of different OmpH-types among Pasteurella multocida isolated from lungs of calves with respiratory problems
| Autor: | Glotov Ag, Vladimir A. Ternovoy, A. O. Sementsova, Glotova Ti, Alexey V. Nefedchenko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype Pasteurella multocida Genotype 040301 veterinary sciences Virulence Factors Cattle Diseases Serogroup Microbiology Polymerase Chain Reaction Virulence factor 0403 veterinary science 03 medical and health sciences Prevalence Animals Respiratory system Allele Pasteurellosis Pneumonic Gene Lung biology Genetic Variation 04 agricultural and veterinary sciences Sequence Analysis DNA biology.organism_classification Virology 030104 developmental biology Infectious Diseases Cattle Bacterial antigen Variants of PCR Bacterial Outer Membrane Proteins |
| Zdroj: | Microbial pathogenesis. 104 |
| ISSN: | 1096-1208 |
| Popis: | Pasteurella multocida is among the most important respiratory pathogens of cattle. Outer-membrane protein (OmpH) constitutes an essential bacterial antigen and is well studied in avian bacterial strains. Studies on isolates from cattle with signs of respiratory disease caused by Pasteurella multocida serotypes A and D have not yet been covered in the literature. The objective of this study was a comparative analysis of the ompH gene sequences from 83 isolates and four Russian reference strains of P. multocida to assign them to the allelic variants of the gene (OmpH-types). In addition, the above P. multocida strains have been characterized on the basis of capsular serotypes and virulence-associated genes. The isolates were classified into the OmpH -types based on allele specific PCR and gene fragment sequencing. The isolates of capsular serotype A have been subdivided into 6 OmpH -types, of which the most common types identified were A1 and A2. All capsular serotype D isolates belong to the same OmpH-type (D1). On 16 of a total of 23 farms all isolates belong to only one OmpH-type, on 4 farms - to 2, and on 3 farms - to three OmpH-types. The tbpA and pfhA genes were found more often in the isolates of capsular group А as compared to capsular group D (p ≤ 0.05). OmpH-types of serogroup А differ significantly (p ≤ 0.05) among themselves by the prevalence of the pfhA and hgbB genes. |
| Databáze: | OpenAIRE |
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