Nanoclay based study on protein stability and aggregation and its implication in human health
| Autor: | Zeba Tarannum, Romana Parveen, Sher Ali, Sadaf Fatima |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Amyloid
Circular dichroism 02 engineering and technology Protein aggregation Biochemistry Polymerization 03 medical and health sciences chemistry.chemical_compound Amyloid disease Structural Biology medicine Humans Molecular Biology Serum Albumin 030304 developmental biology 0303 health sciences biology Protein Stability General Medicine 021001 nanoscience & nanotechnology Human serum albumin Nanostructures Congo red chemistry Chaperone (protein) Bentonite biology.protein Biophysics Clay Muramidase Thioflavin Lysozyme 0210 nano-technology medicine.drug |
| Zdroj: | International Journal of Biological Macromolecules. 166:385-400 |
| ISSN: | 0141-8130 |
| Popis: | Protein aggregation is the major cause of several acute amyloid diseases such as Parkinson's, Huntington's, Alzheimer's, Lysozyme Systemic amyloidosis, Diabetes-II etc. While these diseases have attracted much attention but the cure is still unavailable. In the present study, Human Serum Albumin (HSA) and Human Lysozyme (HL) were used as the model proteins to investigate their aggregations. Nanoclays are hydrous silicates found in clay fraction of soil and known as natural nanomaterials. They have long been used in several applications in health-related products. In the present paper, the different types of nanoclays (MMT K-10, MMT K-30, Halloysite, Bentonite) were used to inhibit the process of HSA and HL aggregation. Aggregation experiments were evaluated using several biophysical tools such as Turbidity measurements, Intrinsic fluorescence, 1-anilino-8-naphthalene sulfonate (ANS), Thioflavin T (Th T), congo red (CR) binding assays and Circular dichroism. Results demonstrated that all the nanoclays inhibit the DTT-induced aggregation. However, bentonite and MMT K-10 were progressively intense and potent as they slowed down nucleation stage which can be perceived using several biophysical techniques. Hence, nanoclays can be used as an artificial chaperone and might provide effective treatment against several protein aggregation related disorders. |
| Databáze: | OpenAIRE |
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