Chondrogenesis of Adipose-Derived Adult Stem Cells in a Poly-Lactide-Co-Glycolide Scaffold
| Autor: | Phillip Niemeyer, Hagen Schmal, Bjoern Stark, Alexander T. Mehlhorn, Norbert P. Südkamp, Guenter Finkenzeller, Jörn Zwingmann, Martin Dauner |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Integrins Transplantation Heterologous Biomedical Engineering Biocompatible Materials Bioengineering Mice SCID Biochemistry Biomaterials Extracellular matrix Mice chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Materials Testing Animals Humans Aggrecans Lactic Acid RNA Messenger Aggrecan DNA Primers Cartilage oligomeric matrix protein Base Sequence Tissue Engineering Tissue Scaffolds biology Chemistry Cell Differentiation Transforming growth factor beta Chondrogenesis Molecular biology Adult Stem Cells PLGA Cartilage Adipose Tissue biology.protein Collagen Stem cell Polyglycolic Acid Biomedical engineering Adult stem cell |
| Zdroj: | Tissue Engineering Part A. 15:1159-1167 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2008.0069 |
| Popis: | Adult adipose-derived stem cells (ASCs) are considered to be an alternative cell source for cell-based cartilage repair because of their multiple differentiation potentials. This article addresses the chondrogenic differentiation of ASCs seeded into poly-lactide-co-glycolide (PLGA) scaffolds after implantation in a subcutaneous pocket of nude mice. Human ASCs were seeded into PLGA (polylactic acid:polyglycolic acid = 90:10) scaffolds and cultured in transforming growth factor beta 1 (TGF-beta1)-containing medium for 3 weeks in vitro. Then specimens were implanted into a subcutaneous pocket of severe combined immunodeficiency mice and harvested after 8 weeks. Chondrospecific messenger RNA (mRNA) expression was analyzed using reverse transcriptase polymerase chain reaction. Corresponding extracellular matrix (ECM) synthesis was demonstrated using immunohistochemical staining. Chondrospecific marker molecules such as collagen type II and type X, cartilage oligomeric matrix protein, and aggrecan subsequently increased during the 3 weeks period in vitro. After a further 8 weeks, in vivo samples pretreated with TGF-beta1 continued expressing collagen type II and aggrecan mRNA, and collagen type II was found within the ECM using immunohistochemistry. Chondrospecific mRNA was not detected in control samples. ASC-seeded PLGA scaffolds express a stable chondrogenic phenotype in a heterotopic model of cartilage transplantation and represent a suitable tool for tissue engineering of cartilage. |
| Databáze: | OpenAIRE |
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