Tenascin-C expression and distribution in cultured human chondrocytes and chondrosarcoma cells
| Autor: | Joel A. Block, Michelle Ghert, Harold P. Erickson, Sean P. Scully, Wen-ning Qi |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Gene isoform
Angiogenesis Blotting Western Alternative splicing Tenascin C Chondrosarcoma Bone Neoplasms Tenascin Matrix (biology) Biology musculoskeletal system medicine.disease Immunohistochemistry Polymerase Chain Reaction Molecular biology Extracellular matrix Chondrocytes Cell culture medicine biology.protein Humans Protein Isoforms Orthopedics and Sports Medicine Cells Cultured |
| Zdroj: | Journal of Orthopaedic Research. 20:834-841 |
| ISSN: | 1554-527X 0736-0266 |
| DOI: | 10.1016/s0736-0266(01)00172-3 |
| Popis: | Tenascin-C (TNC) is an oligomeric glycoprotein of the extracellular matrix with several distinct isoforms variably expressed during embryogenesis, tumorogenesis, angiogenesis and wound healing. In the normal human adult, TNC is found in large concentrations in articular cartilage, suggesting tissue-specific function. The purpose of this study was to determine the specific in vitro TNC splicing patterns of articular chondrocytes and a human chondrosarcoma cell line. Cells were cultured in a three-dimensional bead system and TNC splice variant expression and distribution were examined with the use of Western blotting techniques, semi-quantitative reverse-transcription polymerase chain reaction and immunohistochemistry. At both the transcriptional and post-translational levels, the chondrocytes were found to express significantly higher levels of the smaller 220 kDa isoform (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text