Expression Analysis of Interferon-Stimulated Gene 15 in the Rock Bream Oplegnathus fasciatus against Rock Bream Iridovirus (RSIV) Challenge
| Autor: | Hyun Chul Kim, Seunghyung Lee, Gyeong Eon Noh, In Jung Yang, Kyung-Hee Kim, Woo-Jin Kim, Hyung Kyu Hwang, Choul-Ji Park, Jong-Won Park, Young Mee Lee |
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| Rok vydání: | 2017 |
| Předmět: |
Infectivity
food.ingredient Oplegnathus fasciatus Interferon-stimulated gene Iridovirus Spleen Biology ISG15 Microbiology Original Research Paper medicine.anatomical_structure Immune system food Viral replication Gene expression medicine Rock bream iridovirus (RSIV) IFN-stimulated genes (ISGs) Rock bream |
| Zdroj: | Development & Reproduction |
| ISSN: | 2465-9541 2465-9525 |
| Popis: | Interferon-stimulated gene 15 (ISG15) is known to interfere with viral replication and infection by limiting the viral infection of cells. Interferon-stimulated gene 15 (ISG15) interferes with viral replication and infectivity by limiting viral infection in cells. It also plays an important role in the immune response. In this study, tissue-specific expression of ISG15 in healthy rock bream samples and spatial and temporal expression analysis of rock bream ISG15 (RbISG15) were performed following rock bream iridovirus (RSIV) infection. RbISG15 expression was significantly higher in the eye, gill, intestine, kidney, liver, muscle, spleen, and stomach, but low in the brain. There were particularly high levels of expression in the liver and muscle. RbISG15 expression was also examined in several tissues and at various times following RSIV infection. ISG15 expression increased within 3 h in the whole body and decreased at 24 h after infection. In addition, temporal expression of several tissues following RSIV infection showed a similar pattern in the muscle, kidney, and spleen, increasing at 3 h and decreasing at 72 h. These results suggest that ISG15 plays an important role in the immune response of rock bream. Overall, this study characterizes the response of RbISG15 following RSIV infection. |
| Databáze: | OpenAIRE |
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