A single-cell view on the ecophysiology of anaerobic phototrophic bacteria
| Autor: | Bo Barker Jørgensen, Hannah Halm, Niculina Musat, Sandro Peduzzi, B. Winterholler, Rudolf Amann, Francois Hillion, Peter Hoppe, Marcel M. M. Kuypers, Francois Horreard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Ecophysiology
Time Factors Nitrogen Microorganism Fresh Water Chromatiaceae Chlorobi Bacteria Anaerobic Microbial ecology Botany Biomass Ecosystem Multidisciplinary Phototroph biology Ecology Biological Sciences biology.organism_classification Chromatium okenii Carbon Oxygen Quaternary Ammonium Compounds Phototrophic Processes Microscopy Fluorescence Switzerland Bacteria |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Musat, N, Halm, H, Winterholler, B, Hoppe, P, Peduzzi, S, Hillion, F, Horreard, F, Amann, R, Jørgensen, B B & Kuypers, M M M 2008, ' A single-cell view on the ecophysiology of anaerobic phototrophic bacteria. ', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 46, pp. 17861-17866 . https://doi.org/10.1073/pnas.0809329105 |
| DOI: | 10.1073/pnas.0809329105 |
| Popis: | Quantitative information on the ecophysiology of individual microorganisms is generally limited because it is difficult to assign specific metabolic activities to identified single cells. Here, we develop and apply a method, Halogen In Situ Hybridization-Secondary Ion Mass Spectroscopy (HISH-SIMS), and show that it allows simultaneous phylogenetic identification and quantitation of metabolic activities of single microbial cells in the environment. Using HISH-SIMS, individual cells of the anaerobic, phototropic bacteria Chromatium okenii , Lamprocystis purpurea , and Chlorobium clathratiforme inhabiting the oligotrophic, meromictic Lake Cadagno were analyzed with respect to H 13 CO 3 − and 15 NH 4 + assimilation. Metabolic rates were found to vary greatly between individual cells of the same species, showing that microbial populations in the environment are heterogeneous, being comprised of physiologically distinct individuals. Furthermore, C. okenii , the least abundant species representing ≈0.3% of the total cell number, contributed more than 40% of the total uptake of ammonium and 70% of the total uptake of carbon in the system, thereby emphasizing that numerically inconspicuous microbes can play a significant role in the nitrogen and carbon cycles in the environment. By introducing this quantification method for the ecophysiological roles of individual cells, our study opens a variety of possibilities of research in environmental microbiology, especially by increasing the ability to examine the ecophysiological roles of individual cells, including those of less abundant and less active microbes, and by the capacity to track not only nitrogen and carbon but also phosphorus, sulfur, and other biological element flows within microbial communities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|