Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from Cottonseed Oil and Valeric Acid in Batch Culture of Ralstonia sp. Strain JC-64

Autor: Shin Ono, Choichiro Shimasaki, Masami Inoue, Lixing Zhao, Cunjiang Song
Rok vydání: 2001
Předmět:
Zdroj: Applied Biochemistry and Biotechnology. 94:169-178
ISSN: 0273-2289
Popis: A Ralstonia sp. strain JC-64 that is capable of accumulating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[3HB-co-3HV]) from cottonseed oil and valeric acid was isolated. By using a high limiting-nitrogen (HLN) mineral medium as the medium for the second stage of the fermentation process and by adding the two carbon sources at different times, a range of copolymers with 12–62 mol% of 3HV were produced from a series of HLN mineral mediums containing different compositions of cottonseed oil and valeric acid by Ralstonia sp. JC-64. The melting temperature (T m ) of polyhydroxybutyrate from cottonseed oil was 174°C and that of P(3HB-co-3HV) with the highest 3HV-mol fraction (62%) was 81°C.
Databáze: OpenAIRE