δ-Catenin engages the autophagy pathway to sculpt the developing dendritic arbor
| Autor: | Jyothi Arikkath, Eunju Seong, Cheryl Ligon, Tammy R. Chaudoin, Shilpa Buch, Ethan J. Schroeder, Li Yuan, Stephen J. Bonasera, Yu Cai, Nicholas W. DeKorver |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Delta Catenin Dendrite Biological neuron model Autophagy-Related Protein 7 Hippocampus Biochemistry law.invention Mice 03 medical and health sciences Neurobiology Confocal microscopy law Apical dendrite Autophagy medicine Animals Molecular Biology Cells Cultured Gene knockdown 030102 biochemistry & molecular biology Chemistry Pyramidal Cells Catenins Dendritic Cells Cell Biology Rats Cell biology 030104 developmental biology medicine.anatomical_structure nervous system Gene Knockdown Techniques Catenin Neuron |
| Zdroj: | J Biol Chem |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.ra120.013058 |
| Popis: | The development of the dendritic arbor in pyramidal neurons is critical for neural circuit function. Here, we uncovered a pathway in which δ-catenin, a component of the cadherin-catenin cell adhesion complex, promotes coordination of growth among individual dendrites and engages the autophagy mechanism to sculpt the developing dendritic arbor. Using a rat primary neuron model, time-lapse imaging, immunohistochemistry, and confocal microscopy, we found that apical and basolateral dendrites are coordinately sculpted during development. Loss or knockdown of δ-catenin uncoupled this coordination, leading to retraction of the apical dendrite without altering basolateral dendrite dynamics. Autophagy is a key cellular pathway that allows degradation of cellular components. We observed that the impairment of the dendritic arbor resulting from δ-catenin knockdown could be reversed by knockdown of autophagy-related 7 (ATG7), a component of the autophagy machinery. We propose that δ-catenin regulates the dendritic arbor by coordinating the dynamics of individual dendrites and that the autophagy mechanism may be leveraged by δ-catenin and other effectors to sculpt the developing dendritic arbor. Our findings have implications for the management of neurological disorders, such as autism and intellectual disability, that are characterized by dendritic aberrations. |
| Databáze: | OpenAIRE |
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