New mutations that suppress mating-type vegetative incompatibility in Neurospora crassa
| Autor: | T. S. Vellani, N. L. Glass, A. J. F. Griffiths |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Mating type
Genetic Linkage Genes Fungal Mutant Locus (genetics) law.invention Neurospora crassa law Reproduction Asexual Gene duplication Genetics Allele Genes Suppressor Molecular Biology Alleles Crosses Genetic Heterokaryon biology fungi General Medicine Genes Mating Type Fungal biology.organism_classification Mutation Suppressor Biotechnology |
| Zdroj: | Genome. 37:249-255 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/g94-035 |
| Popis: | The mating-type locus in the ascomycete Neurospora crassa functions as a vegetative-incompatibility locus during asexual growth such that A + a heterokaryons and A/a partial diploids are inhibited in their growth. In this study, we sought mutations that suppress mating-type associated vegetative incompatibility by using A/a partial diploids. Mutants were selected as spontaneous escapes from inhibited growth. Suppressors were identified by selecting escapes that retained the capacity to mate with both A and a strains. The escaped partial diploids were then outcrossed to remove the duplication. Seven suppressors were identified that segregated as single, recessive mutations. All seven of the suppressors were shown to be allelic to a previously obtained suppressor, tol, by segregation analyses and heterokaryon tests. This result indicates that all seven mutations are new alleles of tol and suggests that tol is a key regulatory locus in the expression of mating-type associated vegetative incompatibility in N. crassa.Key words: vegetative incompatibility, mating type, suppressors, Neurospora. |
| Databáze: | OpenAIRE |
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