Antioxidant, haemolytic activities and HPLC–DAD–ESI–MSn characterization of phenolic compounds from root bark of Juniperus oxycedrus subsp. oxycedrus
| Autor: | Fouzia Atik-Bekara, Zahia Boucherit, Choukri Tefiani, Rachid Azzi, Farah Haddouchi, Tarik Mohammed Chaouche, Riadh Ksouri, Romain Larbat |
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| Přispěvatelé: | Université Aboubekr Belkaid - University of Belkaïd Abou Bekr [Tlemcen], Laboratoire Agronomie et Environnement (LAE), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
| Rok vydání: | 2015 |
| Předmět: |
Juniperus oxycedrus
0106 biological sciences DPPH [SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy 01 natural sciences chemistry.chemical_compound Antioxidant activity Root bark Organic chemistry Isorhamnetin Haemolysis activity ABTS Chromatography biology 010401 analytical chemistry biology.organism_classification HPLC-MSn Quercitrin 0104 chemical sciences chemistry Proanthocyanidin visual_art visual_art.visual_art_medium Bark Quercetin Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Industrial Crops and Products Industrial Crops and Products, Elsevier, 2015, 64, pp.182-187. ⟨10.1016/j.indcrop.2014.10.051⟩ |
| ISSN: | 0926-6690 |
| DOI: | 10.1016/j.indcrop.2014.10.051 |
| Popis: | International audience; This study investigates the phenolic compound content antioxidant and haemolytic activities in four extracts (methanol, water, hexane and dichloromethane) of Juniperus oxycedrus subsp. oxycedrus root bark. The methanol extract was the most concentrated in total phenolics (76.1 +/- 2.8 mg GAE/g DW), flavonoids (39 +/- 2.5 mg CE/g DW) and tannins (31.3 +/- 2.1 mg CE/g DW). HPLC-MSn analysis of the methanol extract led to the identification of proanthocyanidin oligomers, quercetin hexose, quercitrin, and isorhamnetin hexose. The highest antioxidant activities were found in the methanol extract which exhibited the lowest IC50 in all the antioxidant assays i.e. DPPH, ABTS, oxygen singlet, hydroxyl radical and superoxide anion scavenging assays, inhibition of the beta-carotene bleaching and lowest EC50 in iron reducing assays. Incubation of the four extracts (20 mg/ml) with human erythrocytes for one hour led to haemolytic activities between 2.05% and 4.37%. The present findings suggest than the root bark could be used as food ingredient. |
| Databáze: | OpenAIRE |
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