tRNA Wobble Modification Affects Leaf Cell Development in Arabidopsis thaliana
| Autor: | Akiko Harada, Kosei Iwabuchi, Takato Yano, Masato Nakai, Gorou Horiguchi, Yumi Nakai, Ikuko Hara-Nishimura |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine TRNA modification Physiology Mutant Arabidopsis Plant Science 01 natural sciences Palisade cell 03 medical and health sciences RNA Transfer Endoreduplication Arabidopsis thaliana RNA Processing Post-Transcriptional biology Chemistry Arabidopsis Proteins Translation (biology) Cell Biology General Medicine biology.organism_classification Cell biology Biosynthetic Pathways Plant Leaves 030104 developmental biology Phenotype RNA Plant Mutation Small Ubiquitin-Related Modifier Proteins Leaf morphogenesis Mesophyll Cells Sulfur 010606 plant biology & botany |
| Zdroj: | Plantcell physiology. 60(9) |
| ISSN: | 1471-9053 |
| Popis: | The tRNA modification at the wobble position of Lys, Glu and Gln (wobbleU* modification) is responsible for the fine-tuning of protein translation efficiency and translation rate. This modification influences organism function in accordance with growth and environmental changes. However, the effects of wobbleU* modification at the cellular, tissue, or individual level have not yet been elucidated. In this study, we show that sulfur modification of wobbleU* of the tRNAs affects leaf development in Arabidopsis thaliana. The sulfur modification was impaired in the two wobbleU*-modification mutants: the URM1-like protein-defective mutant and the Elongator complex-defective mutants. Analyses of the mutant phenotypes revealed that the deficiency in the wobbleU* modification increased the airspaces in the leaves and the leaf size without affecting the number and the area of palisade mesophyll cells. On the other hand, both mutants exhibited increased number of leaf epidermal pavement cells but with reduced cell size. The deficiency in the wobbleU* modification also delayed the initiation of the endoreduplication processes of mesophyll cells. The phenotype of ASYMMETRIC LEAVES2-defective mutant was enhanced in the Elongator-defective mutants, while it was unchanged in the URM1-like protein-defective mutant. Collectively, the findings of this study suggest that the tRNA wobbleU* modification plays an important role in leaf morphogenesis by balancing the development between epidermal and mesophyll tissues. |
| Databáze: | OpenAIRE |
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