Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling
| Autor: | Anna Ratajska, Sławomir Rumiński, Justyna Niderla-Bielińska, Ewa Jankowska-Steifer, Grzegorz Gula, Ewelina Kiernozek, Agnieszka Jasińska, Aleksandra Flaht-Zabost |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Embryonic cardiac macrophages Histology Angiogenesis Neovascularization Physiologic 030204 cardiovascular system & hematology Biology Models Biological Extracellular matrix remodeling Fetal Development Extracellular matrix Mice 03 medical and health sciences 0302 clinical medicine Animals Lymphangiogenesis Molecular Biology Original Paper Macrophages Myocardium Cell Biology Embryonic stem cell Extracellular Matrix Cell biology Mice Inbred C57BL Vascular endothelial growth factor B Medical Laboratory Technology Vascular endothelial growth factor A 030104 developmental biology Lymphatic system Vascular endothelial growth factor C Mice Inbred CBA |
| Zdroj: | Histochemistry and Cell Biology |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-020-01934-1 |
| Popis: | The role of cardiac tissue macrophages (cTMs) during pre- and postnatal developmental stages remains in many aspects unknown. We aimed to characterize cTM populations and their potential functions based on surface markers. Our in situ studies of immunostained cardiac tissue specimens of murine fetuses (from E11to E17) revealed that a significant number of embryonic cTMs (phenotyped by CD45, CD68, CD64, F4/80, CD11b, CD206, Lyve-1) resided mostly in the subepicardial space, not in the entire myocardial wall, as observed in adult individuals. cTMs accompanied newly developed blood and lymphatic vessels adhering to vessel walls by cellular processes. A subpopulation of CD68-positive cells was found to form accumulations in areas of massive apoptosis during the outflow tract remodeling and shortening. Flow cytometry analysis at E14 and E17 stages revealed newly defined three subpopulations:CD64low, CD64highCD206-and CD64highCD206+. The levels of mRNA expression for genes related to regulation of angiogenesis (VEGFa, VEGFb, VEGFc, bFGF), lymphangiogenesis (VEGFc) and extracellular matrix (ECM) remodeling (MMP13, Arg1, Ym1/Chil3, Retlna/FIZZ1) differed among the selected populations and/or embryonic stages. Our results demonstrate a diversity of embryonic cTMs and their tissue-specific locations, suggesting their various potential roles in regulating angiogenesis, lymphangiogenesis and ECM remodeling. |
| Databáze: | OpenAIRE |
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