Establishment of induced pluripotent stem cell (iPSC) line from 55-year old male patient with hemorrhagic Moyamoya disease
| Autor: | Jacopo Zasso, Matteo Marcatili, Fabio Marsoner, Luigi A. Lanterna, Thodoris Karnavas, Marina Cardano, Luciano Conti |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Cellular differentiation Genetic Vectors Induced Pluripotent Stem Cells Karyotype Embryoid body Germ layer Sendai virus Peripheral blood mononuclear cell Cell Line Moyamoya disease 03 medical and health sciences medicine Humans Induced pluripotent stem cell Embryoid Bodies Medicine(all) hiPSCs biology Cell Differentiation Cell Biology General Medicine Middle Aged Cellular Reprogramming biology.organism_classification medicine.disease 030104 developmental biology Microscopy Fluorescence Immunology Leukocytes Mononuclear Moyamoya Disease Transcription Factors Developmental Biology |
| Zdroj: | Stem Cell Research. 17:623-626 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2016.11.006 |
| Popis: | Peripheral blood mononuclear cells (PBMCs) were collected from 55-year old male patient with a confirmed diagnosis of hemorrhagic Moyamoya disease (MMD). PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. A footprint-free hiPSC line was characterized by the expression of pluripotency markers and a normal karyotype. These cells were able to give rise to Embryoid Bodies and to a progeny of differentiated cells belonging to the 3 germ layers. This hiPSC line represents a suitable tool for modelling in vitro MMD disease to investigate the cellular mechanisms underlying the occurrence of this pathology. |
| Databáze: | OpenAIRE |
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