Genetic and Molecular Organization of the Alkylbenzene Catabolism Operon in the Psychrotrophic Strain Pseudomonas putida 01G3
| Autor: | N. Truffaut, Annabelle L. Zgoda, C.-O. Sarde, P. A. Chablain |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Operon
Sequence analysis Molecular Sequence Data Genetics and Molecular Biology Applied Microbiology and Biotechnology Molecular evolution Genes Regulator Benzene Derivatives Cloning Molecular Promoter Regions Genetic Genetics Ecology biology Base Sequence Pseudomonas putida Nucleic acid sequence Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Metabolic pathway Mutagenesis Insertional Biochemistry Genes Bacterial Pseudomonadales DNA Transposable Elements Food Science Biotechnology Pseudomonadaceae |
| Popis: | The 11-kb sequence encompassing the alkylbenzene upper pathway in Pseudomonas putida 01G3, a psychrotrophic strain able to degrade alkylbenzenes at low temperatures, was characterized. Together with a potential regulator (EbdR), six putative enzymes (EbdAaAbAcAdBC) were identified, and they exhibited highly significant similarities with enzymes implicated in the equivalent pathway in P. putida RE204. ebd genes appeared to be preferentially induced by ethylbenzene. Multiple-alignment data and growth rate measurements led us to classify 01G3 and closely related strains in two groups with distinct substrate specificities. Close to identified genes, remnants of IS 5 -like elements provided insight into the evolution of catabolic sequences through rearrangements from a less complex ancestral cluster. |
| Databáze: | OpenAIRE |
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