Radioiodination of mouse anti-III β-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies
Autor: | Helena Svecova, Hana Chaloupkova, Daniel Seifert, Miroslav Fiser, Veronika Kleinova, Jörg Bäurle, Karel Kranda, Vlastimil Král |
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Rok vydání: | 2008 |
Předmět: |
Male
Biodistribution Immunoconjugates medicine.drug_class Monoclonal antibody Iodine Radioisotopes Mice Tubulin medicine Animals Tissue Distribution Radionuclide Imaging Immunoglobulin Fragments Radiation biology Chemistry Antibodies Monoclonal Peripheral Nervous System Diseases Immunohistochemistry Molecular biology Mice Inbred C57BL Isotope Labeling biology.protein Autoradiography Antibody |
Zdroj: | Applied Radiation and Isotopes. 66:310-316 |
ISSN: | 0969-8043 |
DOI: | 10.1016/j.apradiso.2007.09.005 |
Popis: | The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125I in order to develop new imaging agents against the specific neuronal marker III β-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv. |
Databáze: | OpenAIRE |
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