Radioiodination of mouse anti-III β-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies

Autor: Helena Svecova, Hana Chaloupkova, Daniel Seifert, Miroslav Fiser, Veronika Kleinova, Jörg Bäurle, Karel Kranda, Vlastimil Král
Rok vydání: 2008
Předmět:
Zdroj: Applied Radiation and Isotopes. 66:310-316
ISSN: 0969-8043
DOI: 10.1016/j.apradiso.2007.09.005
Popis: The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125I in order to develop new imaging agents against the specific neuronal marker III β-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
Databáze: OpenAIRE