High D-arabitol production with osmotic pressure control fed-batch fermentation by Yarrowia lipolytica and proteomic analysis under nitrogen source perturbation
| Autor: | Suyue Zheng, LiBo Yang, Wei Kong, Danpeng Li, Weina Yang, Shuang Zhao, Yucui Wu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Glycerol
Proteomics biology Chemistry Nitrogen deficiency Nitrogen Yarrowia Bioengineering Pentose phosphate pathway biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Metabolic engineering chemistry.chemical_compound Sugar Alcohols Arabitol Osmotic Pressure Fermentation Osmotic pressure Biotechnology |
| Zdroj: | Enzyme and microbial technology. 152 |
| ISSN: | 1879-0909 |
| Popis: | D -arabitol, a five-carbon sugar alcohol, is widely used in food and pharmacy industry as a lower calorie sweetener or intermediate. Appropriate osmotic pressure was confirmed to facilitate polyol production by an osmophilic yeast strain of Yarrowia lipolytica with glycerol. In this study, an osmotic pressure control fed-batch fermentation strategy was used for high D -arabitol producing by Y. lipolytica ARA9 with crude glycerol. Glycerol was added to the broth quantitatively not only as a substrate but also as an osmotic agent. Meanwhile, NH3·H2O was fed as a nitrogen source and pH regulator. The maximum D -arabitol production reached 118.5 g/L at 108 h with the yield of 0.49 g/g and productivity of 1.10 g/L/h, respectively. Furthermore, a comparative proteomic analysis was used to study the cellular responses under excess and deficient nitrogen sources. Thirty-one differentially expressed protein spots belonging to seven different biological processes were identified. Excess nitrogen source enhanced gluconeogenesis and pentose phosphate pathways, both of which were involved in arabitol synthesis. In addition, cell growth was facilitated by increased expression of nucleotide and structural proteins. Enhanced energy and NADPH biosynthesis were employed to create a reductive environment and quell reactive oxygen species, improving D -arabitol production. Nitrogen deficiency resulted in cell rescue and stress response mechanisms such as reactive oxygen species elimination and heat shock protein response. The identified differentially expressed proteins provide information to reveal the mechanisms of the cellular responses under nitrogen source perturbation, and also provide guidance to improve D -arabitol production in metabolic engineering or process optimization methodologies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect