Field Evaluation of the MUG Assay for Enumerating Escherichia coli in Seawater and Oysters from Southeastern United States

Autor: Miles L. Motes, James T. Peeler
Rok vydání: 2019
Předmět:
Zdroj: Journal of food protection. 54(4)
ISSN: 1944-9097
Popis: Oysters and seawater collected from the southeastern United States were examined for fecal coliforms and Escherichia coli , using the current procedure of the American Public Health Association (APHA) and the fluorogenic 4-methylumbelliferyl-β-D-glucuronide (MUG) modified APHA procedure. After the presence of E. coli in both methods was confirmed by conventional IMViC procedures, there was no significant difference between method means at the α = 0.05 level. In oysters, low confirmation rates of 67 and 77% were observed by the APHA and the MUG methods, respectively. Seawater had the greatest confirmation rates (95%) by the MUG method. The MUG method may be a suitable alternative to the current APHA method for the microbiological evaluation of oysters and seawater.
Databáze: OpenAIRE