Methods to identify and optimize small molecules interacting with RNA (SMIRNAs)
| Autor: | Simon Vezina-Dawod, Matthew D. Disney, Andrei Ursu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pharmacology RNA Untranslated Target engagement RNA Computational biology Biology Microarray Analysis Phenotype Small molecule Article In vitro Small Molecule Libraries 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Human disease 030220 oncology & carcinogenesis Drug Discovery Humans |
| Zdroj: | Drug Discov Today |
| ISSN: | 1359-6446 |
| DOI: | 10.1016/j.drudis.2019.06.019 |
| Popis: | RNAs, particularly noncoding RNAs (ncRNAs), are becoming increasingly important therapeutic targets, because they are causative and antagonists of human disease. Indeed, aberrant RNA structural elements and expression deregulate biological processes. In this review, we describe methodologies to discover and optimize small molecules interacting with RNA (SMIRNAs), including the evaluation of direct target engagement and the rescue of RNA-mediated phenotypes in vitro and in vivo. Such studies are essential to fully characterize the mode of action of SMIRNAs and advance our understanding of rationally and efficiently drugging RNAs for therapeutic benefit. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect