A complete Neandertal mitochondrial genome sequence determined by high-throughput sequencing
| Autor: | Green, Richard E., Malaspinas, Anna-Sapfo, Krause, Johannes, Briggs, Adrian W., Johnson, Philip L. F., Uhler, Caroline, Meyer, Matthias, Good, Jeffrey M., Maričić, Tomislav, Stenzel, Udo, Prüfer, Kay, Siebauer, Michael, Burbano, Hernán A., Ronan, Michael, Rothberg, Jonathan M., Egholm, Michael, Rudan, Pavao, Brajković, Dejana, Kućan, Željko, Gušić, Ivan, Wikström, Må, rten, Laakkonen, Liisa, Kelso, Janet, Slatkin, Montgomery, Svante, Pääbo |
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| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Models Molecular Mitochondrial DNA Sequence analysis Croatia Molecular Sequence Data ChemBio DNA Eco_Evol Neanderthal genome project 010603 evolutionary biology 01 natural sciences Genome DNA Mitochondrial Article General Biochemistry Genetics and Molecular Biology DNA sequencing Bone and Bones Evolution Molecular 03 medical and health sciences Animals Humans Denisovan 030304 developmental biology Whole genome sequencing Genetics mtDNA control region 0303 health sciences biology Base Sequence Biochemistry Genetics and Molecular Biology(all) Fossils ECO_EVOL Hominidae Sequence Analysis DNA biology.organism_classification CHEMBIO Cyclooxygenase 2 Genome Mitochondrial |
| Zdroj: | Cell. 134(3) |
| ISSN: | 1097-4172 |
| Popis: | A complete mitochondrial (mt) genome sequence was reconstructed from a 38,000 year-old Neandertal individual with 8341 mtDNA sequences identified among 4.8 Gb of DNA generated from approximately 0.3 g of bone. Analysis of the assembled sequence unequivocally establishes that the Neandertal mtDNA falls outside the variation of extant human mtDNAs, and allows an estimate of the divergence date between the two mtDNA lineages of 660,000 +/- 140,000 years. Of the 13 proteins encoded in the mtDNA, subunit 2 of cytochrome c oxidase of the mitochondrial electron transport chain has experienced the largest number of amino acid substitutions in human ancestors since the separation from Neandertals. There is evidence that purifying selection in the Neandertal mtDNA was reduced compared with other primate lineages, suggesting that the effective population size of Neandertals was small. |
| Databáze: | OpenAIRE |
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