Comparison of Native Aspirates and Cytological Smears Obtained by EUS-Guided Biopsies for Effective DNA/RNA Marker Testing in Pancreatic Cancer
| Autor: | Eva Traboulsi, Miroslav Zavoral, Bohus Bunganic, Barbora Belsanova, Marek Minarik, Lucie Benesova, Tereza Halkova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Cancer Research Pancreatic ductal adenocarcinoma Tissue Fixation Cytodiagnosis Biology medicine.disease_cause Pathology and Forensic Medicine Specimen Handling Proto-Oncogene Proteins p21(ras) 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Frequency detection Pancreatic cancer medicine Biomarkers Tumor Humans Endoscopic Ultrasound-Guided Fine Needle Aspiration Aged RNA General Medicine DNA Middle Aged medicine.disease Molecular biology digestive system diseases Pancreatic Neoplasms MicroRNAs 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Mutation Female RNA extraction KRAS Kras mutation Carcinoma Pancreatic Ductal |
| Zdroj: | Pathology oncology research : POR. 26(1) |
| ISSN: | 1532-2807 |
| Popis: | We compare two types of pancreatic carcinoma samples obtained by EUS-guided fine needle biopsy (EUS-FNB) in terms of the success rates and clinical validity of analysis of two most commonly investigated DNA/RNA pancreatic cancer markers, KRAS mutations and miR-21 expression. 118 patients with pancreatic ductal adenocarcinoma underwent EUS-FNB. The collected sample was divided, one part was stored in a stabilizing solution as native aspirate (EUS-FNA) and second part was processed into the cytological smear (EUS-FNC). DNA/RNA extraction was followed by analysis of KRAS mutations and miR-21 expression. For both sample types, the yields of DNA/RNA extraction and success rates of KRAS mutation and miRNA expression were evaluated. Finally, the resulting KRAS mutation frequency and miR-21 prognostic role were compared to literature data from tissue resections. The overall amount of isolated DNA/RNA from EUS-FNC was lower compared to the EUS-FNA, average yield 10 ng vs 147 ng for DNA and average yield 164 vs. 642 ng for RNA, but the success rates for KRAS and miR-21 analysis was 100% for both sample types. The KRAS-mutant detection frequency in EUS-FNC was 12% higher than in EUS-FNA (90 vs 78%). The prognostic role of miR-21 was confirmed in EUS-FNC (p = 0.02), but did not reach statistical significance in EUS-FNA (p = 0.06). Although both types of EUS-FNB samples are suitable for DNA/RNA extraction and subsequent DNA mutation and miRNA expression analysis, reliable results with clinical validity were only obtained for EUS-FNC. |
| Databáze: | OpenAIRE |
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