Antigenic characterization of highly pathogenic avian influenza A(H5N1) viruses with chicken and ferret antisera reveals clade-dependent variation in hemagglutination inhibition profiles
| Autor: | Samuel S. Shepard, Tho Dang Nguyen, H. Rogier van Doorn, Juliet E. Bryant, Thanh Long To, Erica Spackman, Diep T. Nguyen, Long Van Nguyen, David E. Wentworth, Dang Nguyen Hoang, Joyce Jones, Munir Iqbal, Sharmi Thor, David F. Burke, Amanda Balish, C. Todd Davis |
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| Přispěvatelé: | Thor, Sharmi [0000-0002-0234-5403], Apollo - University of Cambridge Repository |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Hemagglutination Inhibition Tests Epidemiology Immunology Hemagglutinin Glycoproteins Influenza Virus Chick Embryo Biology medicine.disease_cause Antibodies Viral Microbiology Antigenic drift Article 03 medical and health sciences Species Specificity Virology Drug Discovery Influenza A virus medicine Antigenic variation Animals Clade Phylogeny Poultry Diseases Hemagglutination assay Influenza A Virus H5N1 Subtype Immune Sera Ferrets virus diseases General Medicine Antigenic Variation Influenza A virus subtype H5N1 3. Good health Vaccination 030104 developmental biology Infectious Diseases Vietnam Influenza in Birds Parasitology Female Chickens |
| Zdroj: | Emerging Microbes & Infections |
| ISSN: | 2222-1751 |
| Popis: | Highly pathogenic avian influenza (HPAI) A(H5N1) viruses pose a significant economic burden to the poultry industry worldwide and have pandemic potential. Poultry vaccination against HPAI A(H5N1) viruses has been an important component of HPAI control measures and has been performed in Vietnam since 2005. To systematically assess antigenic matching of current vaccines to circulating field variants, we produced a panel of chicken and ferret antisera raised against historical and contemporary Vietnamese reference viruses representing clade variants that were detected between 2001 and 2014. The antisera were used for hemagglutination inhibition (HI) assays to generate data sets for analysis by antigenic cartography, allowing for a direct comparison of results from chicken or ferret antisera. HI antigenic maps, developed with antisera from both hosts, revealed varying patterns of antigenic relationships and clustering of viruses that were dependent on the clade of viruses analyzed. Antigenic relationships between existing poultry vaccines and circulating field viruses were also aligned with in vivo protection profiles determined by previously reported vaccine challenge studies. Our results establish the feasibility and utility of HPAI A(H5N1) antigenic characterization using chicken antisera and support further experimental and modeling studies to investigate quantitative relationships between genetic variation, antigenic drift and correlates of poultry vaccine protection in vivo. |
| Databáze: | OpenAIRE |
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